Combinatorial Peptide Library Protocols - Methods in Molecular Biology 1998 edition

Marc Notes: Includes bibliographical references and index. Table of Contents: Synthesis of a One-Bead One-Compound Combinatorial Peptide Library Kit S. Lam and Michal Lebl. Enzyme-Linked Colorimetric Screening of a One-Bead One-Compound Combinatorial Library Kit S. Lam. Synthesis and Screening of Positional Scanning Combinatorial Libraries Colette T. Dooley and Richard A. Houghten. Synthesis and Screening of Peptide Libraries on Continuous Cellulose Membrane Supports Achim Kramer and Jens Schneider-Mergener. Peralkylation: 'Libraries from Libraries': Chemical Transformation of Synthetic Combinatorial Libraries John M. Ostresh, Barbara Dorner, and Richard A. Houghten . Introduction to Combinatorial Solid-Phase Assays for Enzyme Activity and Inhibition Morten Meldal. Preparation of Biocompatible Resins for Library Syntheses Morten Meldal. Intramolecular Fluorescence-Quenched Substrate Libraries Morten Meldal. The Solid-Phase Enzyme Inhibitor Library Assay Morten Meldal. Determination of Peptide Substrate Motifs for Protein Kinases Using a 'One-Bead One Compound' Combinatorial Library Approach Kit S. Lam. The Use of Peptide Library for the Determination of Kinase Peptide Substrates Zhou Songyang and Lewis C. Cantley. Analysis of Protein Kinase Substrate Specificity by the Use of Peptide Libraries on Cellulose Paper (SPOT-Method) Werner J. Tegge and Ronald Frank. Generation of Multiuse Peptide Libraries for Functional Screenings Channa K. Jayawickreme, Shiranthi P. Jayawickreme, and Michael R. Lerner. Functional Screening of Multiuse Peptide Libraries Using Melanophore Bioassay Channa K. Jayawickreme, Shiranthi P. Jayawickreme, and Michael R. Lerner. The Basic Structure of Filamentous Phage and Its Use in the Display of Combinatorial Peptide Libraries Shmuel Cabilly. Construction and Use of a 20-mer Phage Display Epitope LibraryBaruch Stern and Jonathan M. Gershoni. Construction of Disulfide-Constrained Random Peptide Libraries Displayed on Phage Coat Protein VIII Alessandra Luzzago and Franco Felici . Conformational MimicryThrough Random Constraints Plus Affinity Selection Guangming Zhong. The Use of Combinatorial Libraries to Identify Ligands That Interact with Surface Receptors in Living Cells Shmuel Cabilly, Judith Heldman, Eliahu Heldman, and Ephraim Katchalski-Katzir. Screening Phage Display Peptide Libraries on Nitrocellulose Membranes Shmuel Cabilly, Judith Heldman, and Ephraim Katchalski-Katzir. Identification of Disease-Specific Epitopes Atonella Folgori, Alessandra Luzzago, Paolo Monaci, Alfredo Nicosia, Riccardo Cortese, and Franco Felici. Identification of Peptide Ligands for the Antigen Binding Receptor Expressed on Human B-Cell Lymphomas Markus F. Renschler, William J. Dower, and Ronald Levy. Major Histocompatibility Complex Allele-Specific Peptide Libraries and Identification of T-Cell Mimotopes Marc A. Gavin and Michael J. Bevan. Phage Display of Peptide Libraries on Protein Scaffolds Henry B. Lowman. Displaying Libraries on Conformationally Constrained Peptides on the Surface of Escherichia coli as Flagellin Fusions Zhijian Lu, Brian C. Tripp, and John M. McCoy. Combinatorial Peptide Library as an Immunogen J. Estaquier, J.-C. Ameisen, C. Auriault, C. Boutillon, H. Gras-Masse, and A. Tartar. Index."Publisher Marketing: Presents a wide range of elementary techniques needed for the construction and use of peptide libraries. This text describes the various methods of chemical synthesis of peptide libraries and their use in the screening of various acceptor proteins for cognate ligands, as well as screening of enzymes for substrates and inhibitors. Biological libraries that are displayed over the coat protein of filamentous phage or on the surface of bacteria are also covered, as are some of the possible practical applications of combinatorial library technology. State-of-the-art techniques combined with step-by-step instructions should make this book a useful tool in the selection of peptides suitable for drug development.

Contributor Bio:  Cabilly, Shmuel Cabilly, Weizmann Institute of Science, Rehovot, Israel.