Gene Isolation and Mapping Protocols - Methods in Molecular Biology 1997 edition

Marc Notes: Includes bibliographical references and index. Review Quotes: ...a useful addition to the libraries of individuals and institutions... Three Stars. - Doody's Health Sciences Book Review Journal ."..this series of excellent and detailed lab manuals....of general use to all those mapping and isolation genes."-SGM QuarterlyTable of Contents: Gene Mapping Goes from FISH to Surfing the Net, John Valdes and Danilo A. Tagle. Linkage Analysis of Genetic Disorders, Eugene W. Taylor, Jianfeng Xu, Ethylin Wang Jabs, and Deborah A. Meyers. Gene Ordering and Localization by Linkage Analysis, R. E. March. Gene Mapping Using Somatic Cell Hybrids, David P. Kelsell and Nigel K. Spurr. Gene Mapping by FISH, Rafael Espinosa III and Michelle M. Le Beau. Probe Ordering and Distancing by FISH, Marian Kroef, Hans Dauwerse, and Jim Landegent. Physical Mapping by Pulsed-Field Gel Electrophoresis, John Maule. Construction and Use of YAC Contigs in Disease Regions, Stephen Horrigan and Carol Westbrook. Construction and Use of Cosmid Contigs, Nicholas Fairweather. Use of Dinucleotide Polymorphism Analyses in Physical Mapping, Jeff Fairman and Lalitha Nagarajan. Mapping Expressed Sequence Tags (ESTs) by Multiplexing PCR Reactions from Hybrid Cell Panels and Detecting Fluorescently Labeled Products, A. Scott Durkin, Donna R. Maglott, and William C. Nierman. Gene Isolation by Exon Trapping, David B. Krizman. Isolation of Coding Sequences from Genomic Regions Using Direct Selection, Richard G. Del Mastro and Michael Lovett. Isolation of cDNAs Using the YAC Hybridization Screen Method, Carrie Fidler and Jacqueline Boultwood. Detection and Isolation of Differentially Expressed Genes by Differential Display, Weimin Zhu and Peng Liang. Chemical Crosslinking Subtraction (CCLS), Ian N. Hampson and Lynne Hampson. Gene Mapping and Isolation: Access to Databases, Martin J. Bishop. Software for Genetic Linkage Analysis: An Update, Stephen P. Bryant. Exon Detection by Similarity Searches, Jean-Michel Claverie. Index. Publisher Marketing: Designed for researchers wishing to learn the techniques applied by experts in gene mapping. The text includes sufficient background material and covers the major techniques, including the localization of genes by FISH and linkage analysis, gene isolation by YAC hybridization, direct selection, exon trapping and differential display, as well as methods for gene ordering and identification.