Protein-Protein Interactions: Methods and Applications - Methods in Molecular Biology 2nd ed. 2015 edition

Jacket Description/Back: In recent years, our understanding of the protein-protein interaction landscape has expanded significantly. Growing interest in analyzing and targeting protein-protein interactions has resulted in an increased need for detailed methodologies to interrogate and monitor protein-protein interactions. To meet this need, "Protein-Protein Interactions: Methods and Applications" has been updated and expanded. The second edition includes core technological platforms used to study protein-protein interactions, and cutting-edge technologies that reflect recent scientific advances and the emerging focus on therapeutic discovery. Written in the highly successful "Methods in Molecular Biology" series format, chapters include introductions to their respective topics, lists of necessary materials and reagents, step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. These well-detailed protocols describe methods for identifying protein-protein interaction partners, analyzing protein-protein interactions quantitatively and qualitatively, monitoring protein-protein interactions in live cells, and predicting and determining interaction interfaces. Authoritative and cutting-edge, "Protein-Protein Interactions: Methods and Applications, Second Edition" is a valuable resource that will enable readers to elucidate the mechanisms of protein-protein interactions, determine the role of these interactions in diverse biological processes, and target protein-protein interactions for therapeutic discovery. "Table of Contents: PART I OVERVIEWS 1 Structural basis of protein-protein interactions"Robert Liddington"2 Quantitative analysis of protein-protein interactions "Ziad Eletr and Keith Wilkinson"3 Protein-protein interaction databases "Damian Szklarczyk and Lars Juhl Jensen "4 Computational prediction of protein-protein interactions "Tobias Ehrenberger, Lewis C. Cantley, and Michael B. Yaffe"5 Structure-based computational approaches for small-molecule modulation of protein-protein interactions"David Xu, Bo Wang, and Samy O. Meroueh"6 Targeting protein-protein interactions for drug discovery "David C. Fry" PART II LABEL-FREE BIOSENSORS AND TECHNIQUES 7 Studying protein-protein interactions using surface plasmon resonance"Zenata Nikolovska-Coleska"8 Resonant waveguide grating for monitoring bimolecular interactions "Meng Wu and Min Li "9 Quartz microbalance technology for probing biomolecular interactions "Gabriella T. Heller, Alison R. Mercer-Smith, and Malkiat S. Johal"10 Label-free kinetic analysis of an antibody-antigen interaction using biolayer interferometry "Sriram Kumaraswamy and Renee Tobias"11 Characterization of protein-protein interactions by isothermal titration calorimetry"Adrian Velazquez-Campoy, Stephanie A. Leavitt, and Ernesto Freiere"12 Sedimentation equilibrium studies "Ian A. Taylor, John F. Eccleston, and Katrin Rittinger"13 Detecting protein-protein interactions by gel filtration chromatography"Yan Bai"14 Using light scattering to determine the stoichiometry of protein complexes "Jeremy Mogridge"15 Circular dichroism (CD) analyses of protein-protein interactions "Norma Greenfield"16 Protein-protein interaction analysis by nuclear magnetic resonance spectroscopy "Peter M. Thompson, Moriah R. Beck, and Sharon L. Campbell"17 Quantitative protein analysis by mass spectrometry "Vishwajeeth Pagala, ""Anthony High"," ""Xusheng Wang, Haiyan Tan, Kiran Kodali, Ashutosh Mishra"," "" Kanisha Kavdia"," ""Yanji Xu, Zhiping Wu, and Junmin Peng"18 Using peptide arrays created by the SPOT method for defining protein-protein interactions"Yun Young Yim, Katherine Betke, and Heidi Hamm" PART III TAG/AFFINITY-BASED METHODS 19 Fluorescence polarization assay to quantify protein-protein interactions "Ronald T. Raines"20 Forster resonance energy transfer (FRET) microscopy for monitoring biomolecular interactions "Alexa L. Mattheyses and Adam I. Marcus"21 Utilizing ELISA to monitor protein-protein interactions"Zusen Weng and Qinjian Zhao"22 Glutathione-S-transferase (GST)-fusion based assays for studying protein-protein interactions "Haris G. Vikis and Kun-Liang Guan"23 Hexahistidine (6xHis) fusion-based assays for protein-protein interactions "Mary C. Puckett"24 Studying protein-protein interactions via blot overlay/Far Western blot "Randy A. Hall"25 Co-immunoprecipitation from transfected cells "Yoshinori Takahashi"26 "In vivo" protein cross-linking "Fabrice Agou and Michel V""eron" PART IV CELL-BASED BIMOLECULAR INTERACTION REPORTER ASSAYS 27 Identification of protein-protein interactions by standard Gal4p-based yeast two-hybrid screening"Jeroen Wagemans and Rob Lavigne"28 Reverse two-hybrid techniques in the yeast "Saccharomyces cerevisiae" "Matthew A. Bennett, Jack F. Shern, and Richard A. Kahn "29 MAPPIT, a mammalian two-hybrid method for in-cell detection of protein-protein interactions"Irma Lemmens, Sam Lievens, and Jan Tavernier"30 Bioluminescence resonance energy transfer to detect protein-protein interactions in live cells "Nicole E. Brown, Joe B. Blumer, and John R. Hepler"31 Mapping biochemical networks with protein fragment complementation assays "Ingrid Remy and Stephen W. Michnick"32 Detection of protein-protein interaction using bimolecular fluorescence complementation assay"Cau D. Pham"33 Split-luciferase complementation assay to detect channel-protein interactions in live cells "Alexander S. Shavkunov, Syed R. Ali, Neli I. Panova-Elektronova, and Fernanda Laezza"""34 Confocal microscopy for intracellular co-localization of proteins "Toshiyuki Miyashita" PART V HIGH THROUGHPUT SCREENING ASSAYS FOR PROTEIN- PROTEIN INTERACTIONS - CASE STUDIES 35 Fluorescence polarization assay to quantify protein-protein interactions in an HTS format"Yuhong Du"36 Estrogen receptor alpha/co-activator interaction assay - TR-FRET "Terry W. Moore, Jillian R. Gunther, and John A. Katzenellenbogen"37 High content screening biosensor assay to identify disruptors of p53-hDM2 protein-protein interactions"Yun Hua, Christopher J. Strock. and Paul A. Johnston"38 Discovery of inhibitors of the MDM2-p53 protein-protein interaction"Liu Liu, Denzil Bernard, and Shaomeng Wang"39 Biophysical methods for identifying fragment-based inhibitors of protein-protein interactions"Samuel J. Pfaff, Michael S. Chimenti, Mark J. S. Kelly, and Michelle R. Arkin""Publisher Marketing: The second edition covers a wide range of protein-protein interaction detection topics. "Protein-Protein Interactions: Methods and Applications "focuses on core technological platforms used to study protein-protein interactionsand cutting-edge technologies that reflect recent scientific advances and the emerging focus on therapeutic discovery. Written in the highly successful "Methods in Molecular Biology" series format, chapters include introductions to their respective topics, lists of necessary materials and reagents, step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. These well-detailed protocols describe methods for identifying protein-protein interaction partners, analyzing of protein-protein interactions quantitatively and qualitatively, monitoring protein-protein interactions in live cells, and predicting and determining interaction interfaces. Authoritative and cutting-edge, " Protein-Protein Interactions: Methods and Applications, Second Edition "is a valuable resource that will enable readers to elucidate the mechanisms of protein-protein interactions, determine the role of these interactions in diverse biological processes, and target protein-protein interactions for therapeutic discovery."

Contributor Bio:  Fu, Haian Haian Fu is a Professor of Pathology, Hematology, and Medical Oncology at Emory University School of Medicine. He also serves as director of the Chemical Biology Discovery Center and co-director of the Discovery and Developmental Therapeutics Program at the Winship Cancer Institute at Emory University. His research focuses on protein-protein interactions in signal transduction pathways that control cell survival and death in normal and cancer cells. He serves on the editorial board of several scientific journals, including Current Chemical Genomics. He edited Protein-Protein Interactions: Methods and Applications (2004). Dr Fu has been recognized by various honors including the PhRMA Faculty Development Award, the Burroughs Wellcome Fund New Investigator Award, the GRA Distinguished Investigator Award and the Georgia Cancer Coalition Distinguished Cancer Scholar. He is also a recipient of the Award for Excellence in Mentorship by the Emory Graduate Division of Biological and Biomedical Sciences (2000) and the Emory Pharmacology Teaching Excellence Award (2006).