Marion Espeli

T follicular Helper Cells: Methods and Protocols - Methods in Molecular Biology Marion Espeli 2015 edition

Jacket Description/Back: This volume brings together the skills and protocols of numerous laboratories that are at the heart of investigation into the biology of Tfh cells in both mice and humans. As a volume in the highly successful "Methods in Molecular Biology" series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Concise and easy-to-use," T follicular Helper Cells: Methods and Protocols" provides scientist with techniques and protocols that have facilitated breakthroughs in Tfh biology and to present them in a way that will enable both new and experienced researchers to continue to move this exciting field forward."Table of Contents: Part I: Mouse1. Identification of Mouse T Follicular Helper Cells by Flow CytometryAlexandre Meli & Irah King2. Identification of Follicular T Helper Cells in Tissue SectionsYanna Ding, John D. Mountz, and Hui-Chen Hsu3. Tracking Early T Follicular Helper Cell Differentiation In vivoDirk Baumjohann and K. Mark Ansel4. Tracking by Flow Cytometry Antigen-Specific Follicular Helper T-Cells In Wild-Type Animals After Protein Vaccination Svetoslav Chakarov and Nicolas Fazilleau5. Retroviral Vector Expression in TCR Transgenic CD4+ T CellsYoun Soo Choi and Shane Crotty6. Two-photon Microscopy For Imaging Germinal Centres and T Follicular Helper Cells Menna R. Clatworthy7. "In vivo" Induction of T-follicular Helper Cells by Modulation of Regulatory T Cell Function James B. Wing and Shimon Sakaguchi8. Assessing T Follicular Helper Cell Function "in vivo" Antigen-specific B Cell Response to Hapten and Affinity MaturationJessica Natt and Marion Espeli9. The SWHEL System For High-resolution Analysis of "in vivo" Antigen-specific T-Dependent B Cell ResponsesR. Brink, D. Paus, K. Bourne, J. R. Hermes, S. Gardam, T. G. Phan, and T. D. Chan10. Triggering Positive Selection of Germinal Center B cells by Antigen Targeting to DEC-205Giulia Pasqual, Alessandro Angelini, and Gabriel D. Victora11. Detection of Mouse Natural Killer T Follicular Helper (NKTFH) Cells by Flow CytometryPheh-Ping Chang and Carola G. Vinuesa12. Identification of Foxp3+ T Follicular Regulatory (Tfr) Cells by Flow CytometryAna Raquel Maceiras and Luis Graca13. In vitro Assay to Sensitively Measure Tfr Suppressive Capacity and Tfh Stimulation of B Cell ResponsesPeter T. Sage and Arlene H. SharpePart II: Human14. Flow Cytometric Detection and Isolation of Human Tonsil or Lymph Node T Follicular Helper CellsJan Misiak, Karin Tarte, and Patricia Ame-Thomas15. Human Tfh and Tfr cells: Identification and Assessment of their Migration PotentialChang H. Kim, Seika Hashimoto-Hill, and Seung G. Kang16. Analysis of Human Blood Memory T Follicular Helper Subsets Salah-Eddine Bentebibel, Clement Jacquemin, Nathalie Schmitt, and Hideki Ueno17. Flow Cytometric Analysis of Circulating Follicular Helper T (Tfh) and Follicular Regulatory T (Tfr) Populations in Human BloodYunbo Wei, Jinghong Feng, Zhaohua Hou, Xin Maggie Wang, and Di Yu18. Quantifying Helper Cell Function of Human TFH Cells "in vitro"Nina Chevalier"