Pyrosequencing Protocols - Methods in Molecular Biology 2007 edition

Marc Notes: Includes bibliographical references and index. Table of Contents: Table of contents Chapter 1: The History of Pyrosequencing(r). Pal Nyren Chapter 2: Pyrosequencing(r) applications. Sharon Marsh Chapter 3: Web-Based Primer Design Software for Genome Scale Genotyping by Pyrosequencing(r). Steven Ringquist, Christopher Pecoraro, Ying Lu, Alexis Styche, William A. Rudert, Panayiotis V. Benos and Massimo Trucco Chapter 4: Pyrosequencing(r): A Simple Method for Accurate Genotyping. Cristi R. King and Tiffany Scott-Horton Chapter 5: Universal Primer Applications for Pyrosequencing(r). Dong-chuan Guo and Dianna M. Milewicz Chapter 6: Allele Quantification and DNA Pooling Methods. Jonathon Wasson Chapter 7: Multiplex Pyrosequencing(r) for DNA Variation Analysis. Pritesh Patel, Yih-Horng Shiao, and Paolo Fortina Chapter 8: Analysis of gene-specific DNA methylation patterns by the Pyrosequencing(r) technology. Jorg Tost and Ivo Glynne Gut Chapter 9: Detecting mutations that confer oxazolidinone resistance in Gram-positive bacteria. Neil Woodford, Sarah E. North and Matthew J. Ellington Chapter 10: Pyrosequencing(r) Based Strategies for Improved Allele Typing of HLA Loci. Steven Ringquist, Alexis Styche, William A. Rudert, and Massimo Trucco Chapter 11: Pyrosequencing(r) of Phage Display Libraries for the Identification of Cell Specific Targeting Ligands. Ahad A. Rahim Chapter 12: Gene Copy Number Detection in Animal Studies. Gerli Pielberg and Leif Andersson Chapter 13: Detection of Allelic Imbalance in Gene Expression Using Pyrosequencing(r). Hua Wang and Steven C. Elbein Chapter 14: Pyrosequencing(r) Genotype Storage Techniques. Derek J. VanPublisher Marketing: Within Pyrosequencing Protocols, the protocols for utilizing Pyrosequencing technology are described in detail, including trouble-shooting tips and background information. Chapter 1 provides an introduction to the fascinating origins of the methodology. Chapter 2 provides a brief overview of some of the applications Pyrosequencing is used for. Chapters 3 and 4 describe primer selection and the basic technique. Chapters 5 through 7 provide methods for improving the throughput and decreasing the cost of Pyrosequencing. Detailed applications for the technique can be ound in Chapters 8-13, whilst the important aspect of data storage is discussed in Chapter 14. Review Citations:

Scitech Book News 03/01/2007 pg. 67 (EAN 9781588296450, Hardcover)