Afrin Larifa

Efficacy of Trichoderma Against Vector Mosquitoes Afrin Larifa

Larvicidal efficiency of the spore, crude toxin and fractions of Trichoderma reesei was evaluated against vector mosquitoes such as Culex quinquefasciatus, Anopheles stephensi and Aedes aegypti. The study revealed that, the lowest LC50 and LC90 of the spore,crude toxin and fractions are 0.002, and 46, spore ml-1, 11.18ppm and 42.98ppm 9.19ppm and 27.50ppm respectively. Besides, histopathological study unveiled the degeneration as well as alteration in the midgut epithelial cell was observed. Finally, the highly active F3 fraction was identified as T2 toxin, a Trichothecene derivative of Trichoderma sp based on the fraction and GC-FID analysis.